Mechanisms Regulating Stemness and Differentiation in Embryonal Carcinoma Cells
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چکیده
منابع مشابه
Mechanisms Regulating Stemness and Differentiation in Embryonal Carcinoma Cells
Just over ten years have passed since the seminal Takahashi-Yamanaka paper, and while most attention nowadays is on induced, embryonic, and cancer stem cells, much of the pioneering work arose from studies with embryonal carcinoma cells (ECCs) derived from teratocarcinomas. This original work was broad in scope, but eventually led the way for us to focus on the components involved in the gene r...
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BACKGROUND AND OBJECTIVES We investigated the effects of different concentrations of serum, 5-azacytidine, and culture time on the cardiomyogenic differentiation of P19 embryonal carcinoma stem cells in the course of developing an efficient protocol for generating the cardiomyogenic lineage. MATERIALS AND METHODS P19 cells were plated at a density of 1x10(6) cells on 10-cm bacterial dishes fo...
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Two-dimensional electrophoresis was used to find changes in protein synthesis occurring as pluripotent embryonal carcinoma (EC) cells differentiate to give embryoid bodies in vitro. 2-D patterns from other embryonic cell lines, and from the inner cell mass (ICM) cells of mouse embryos, were also analysed for the expression of those proteins showing some change during embryoid body formation and...
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Introduction: The p19 line of embryonal carcinoma cells develops into neurons, astroglia and fibroblasts after aggregation and exposure to retinoic acid (RA). Dehydroepiandroesteron (DHEA) is a neurosteroid, can increase proliferation of human neural stem cell (NSC) and positively regulated the number of neurons produced. This study was initiated to assess the effect of DHEA on neural progenito...
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Retinoids cause differentiation in embryonal carcinoma (EC) cells, thus mimicking events in mammalian development. Here, we show that retinoids also cause apoptosis in P19 EC cells. Characteristic DNA fragmentation was observed within 36 h after addition of retinoic acid (RA). Synthetic retinoids that are selective for RA receptors (RAR) were also effective in inducing apoptosis, whereas RXR se...
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ژورنال
عنوان ژورنال: Stem Cells International
سال: 2017
ISSN: 1687-966X,1687-9678
DOI: 10.1155/2017/3684178